To explore novel soil Streptomyces in Nepal in pursuit of potential antibiotics using Biochemical and Molecular Methods.
Funding Agency: The World Academy of Sciences (TWAS)
Project Introduction: Streptomycetes are masters of secondary metabolism. Besides antibiotics, diverse natural products produced by Streptomyces possess a variety of pharmacological activities including antitumor, antifungal, antiparasitic and immunosuppressant activities, and are used in clinical and veterinary medicine. Actinomycetes had yielded ~3,000 known antibiotics, 90% of those from Streptomyces. The worldwide emergence of multidrug resistant pathogens has become a major therapeutic problem. Moreover, there has been an alarming increase in the incidence of new and re-emerging infectious diseases. Thus, in light of the evidence of rapid global spread of resistant pathogens and new infectious diseases, the need of finding new therapeutic agents against these diseases is of paramount importance.
The distribution of Streptomyces, well known for their secondary metabolite production, is believed to be influenced by soil geochemistry and local microbial community composition. Nepal has large variation in soil type and its contents due to large geographic variation. As a consequence, abundance and the distribution of Streptomyces are also variable with diverse morphology, physiology, and biochemical activities. However, awareness of these facts and studies on enumeration, isolation and screening of actinomycetes for potential bio-activity has so far remained limited in Nepal.This study aims to investigate Streptomyces complexes in various soil biotopes of Nepal and to explore the drugs/antibiotics production potentials of these indigenous Streptomyces.