Isolation and characterization of soil bacteria (Streptomyces spp) as a source of novel bioactive metabolites from different location of Kathmandu
The search for novel and useful bioactive compounds from the ubiquitous Streptomyces has been the main focus of our research. We started by collecting seven soil samples from three locations (Godawari, Teku dumping site and Bagmati riverbank) completely different in texture, geographic condition, moisture. A total of 31 strains were isolated that showed the closest resemblance in appearance to Streptomyces sp. and further characterized by morphological, physiological and biochemical analyses. Each of the isolates was found to be gram positive and capable of producing catalase enzyme. For carbohydrate utilization test, four sugars were used i.e. Sucrose, Glucose, Raffinose and Galactose. Out of thirty one isolates; twenty eight strains were able to utilize Sucrose, twenty seven for Glucose, twenty six for Raffinose and all for Galactose. Twenty three isolates tested positive for Starch hydrolysis test and for the rest, result was ambiguous.