Investigation of pathogenic bacteria and their biofilm composition in oral cavity of Nepalese population from different geographic location and analysis of its impacts on distribution, genetic variation, and pathogenic potential.
Funding Agency: The World Academy of Sciences (TWAS)
Project Introduction: Poor oral cavity hygiene is the major cause of the many dental related health issues. In order to develop efficient oral cavity cleaning products, there should be enough scientific information about the oral microbial diversity in biofilm and its chemical composition. Such scientific data is not available here for Nepalese population. Due to various geographic variations and multiple ethnic populations with multiple cultures and living customs, different oral microbial composition must be obtained. In the present research project, we will investigate oral microbial diversity in Nepalese population from various altitudinal locations, ethnic groups, and various age groups. In addition, genetic variation of microbial species with respect to geographical location and their biochemical and physiological variation will be investigated. We will also investigate chemical secretion of microbial flora in the oral biofilm by using LC-MS and GC-MS analysis. Similarly, microbial enzyme production will also be explored. The co-relation between the oral disease and microbial diversity along with chemical composition of bio-film and extracellular enzymes secreted by microbial species will be investigated. The overall scientific data will pave the way to describe the distribution, pathogenic potential, physiology and genetic variation of pathogenic oral micro-organisms in Nepalese communities. Such information would pave the way to design and produce proper and effective oral cleaning products commercially.